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PhilRice develops method for early rice viral infection detection.
Page 1 of 1
PhilRice develops method for early rice viral infection detection.
The Philippine Institute of Rice Research (PhilRice)
is developing a method under the Biotechnology
Program of the Department of Agriculture (DA)
which can help farmers to detect infections
caused by viruses in rice plants even before
symptoms appear. Normally farmers search of rice virus infections by
visually inspecting the plants for symptoms. But
symptoms occur only after the virus has started
infecting the crop already. Therefore, methods to
detect infections much earlier are said to be
useful for farmers. The method known as Loop Mediated Isothermal Amplification (LAMP), has
already been successful in diagnosing pathogens
in animals and humans. It is reportedly more user
friendly, relatively faster and accurate compared
to earlier biotechnology methods for detecting
rice viruses. Preliminary LAMP results show that rice viruses can be detected a day after
inoculation (DAI) unlike to ELISA's, where three
DAIs (day after inoculation) is require. Further
research reveals that LAMP was also able to detect
the rice ragged stunt virus (RRSV) in the brown
planthopper (BPH). This means that the method is likewise capable of detecting the virus even
before the disease actually happens and even
without a standing rice crop. Other focuses of the
study include the rice tungro spherical virus
(RTSV), rice grassy stunt virus (RGSV) and the rice
dwarf virus (RDV) and its green leafhopper vector. It is hoped that through this fast and accurate
diagnosis of rice diseases, timely pest
management systems will be delivered and costs
from misuse and expenditure of pesticides will be
reduced. The PhilRice began the project to develop the
LAMP method in August 2012 and is expected to
complete within this year or early next year. They
said the final results of the project are interesting
as the method would eventually lead to reduction
of pesticide costs.
For details:
http://www.bic.searca.org/press_releases/2015/03july.html
is developing a method under the Biotechnology
Program of the Department of Agriculture (DA)
which can help farmers to detect infections
caused by viruses in rice plants even before
symptoms appear. Normally farmers search of rice virus infections by
visually inspecting the plants for symptoms. But
symptoms occur only after the virus has started
infecting the crop already. Therefore, methods to
detect infections much earlier are said to be
useful for farmers. The method known as Loop Mediated Isothermal Amplification (LAMP), has
already been successful in diagnosing pathogens
in animals and humans. It is reportedly more user
friendly, relatively faster and accurate compared
to earlier biotechnology methods for detecting
rice viruses. Preliminary LAMP results show that rice viruses can be detected a day after
inoculation (DAI) unlike to ELISA's, where three
DAIs (day after inoculation) is require. Further
research reveals that LAMP was also able to detect
the rice ragged stunt virus (RRSV) in the brown
planthopper (BPH). This means that the method is likewise capable of detecting the virus even
before the disease actually happens and even
without a standing rice crop. Other focuses of the
study include the rice tungro spherical virus
(RTSV), rice grassy stunt virus (RGSV) and the rice
dwarf virus (RDV) and its green leafhopper vector. It is hoped that through this fast and accurate
diagnosis of rice diseases, timely pest
management systems will be delivered and costs
from misuse and expenditure of pesticides will be
reduced. The PhilRice began the project to develop the
LAMP method in August 2012 and is expected to
complete within this year or early next year. They
said the final results of the project are interesting
as the method would eventually lead to reduction
of pesticide costs.
For details:
http://www.bic.searca.org/press_releases/2015/03july.html
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